High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR

Human papillomavirus (HPV) is known to be associated with both cervical and oropharyngeal cancers, yet little are about the co-occurrence of similar HPV subtypes in these subsites. With changing sexual behaviour it necessary evaluate risk a woman abnormal cervix developing cancer oropharynx. A study evaluating infection oral mucosa therefore needed understand 2 different sites Oral wash specimens were collected from total 100 women aged 20-60 years who visited for routine Pap test. DNA was extracted then subjected an house real-time PCR conventional nested detection high 16/18 subtypes. limit established using serially diluted positive cell lines sensitivity specificity assays evaluated The prevalence HPV-16/18 samples (19.7% vs 6.2%) (16.6% nil) higher as detected by our comparison PCR. found 25% SCC Due there possibility that HPV-related lesions can easily transfer virus partner or even self-inoculate which might lead development head & neck turning into cancer.